Coding

Part:BBa_K2116001:Design

Designed by: Asli Azizoglu   Group: iGEM16_ETH_Zurich   (2016-10-13)


Transcriptional regulator esaR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The initial addgene sequence has an internal stop codon and thus noncoding regions, which were removed during construction. Has a T-->Y mutation at position 31, which doesn't effect it's function as far as we could determine.


Source

The original source is the plant pathogen []. We have obtained the D91G variant from Cynthia Collins through Addgene (Addgene plasmid # 47646).

References